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BioModels Database: Quick tour

BioModels Database: Quick tour from http://www.ebi.ac.uk/training/online/course/biomodels-database-quick-tour.

Keywords: Systems

BioModels Database: Quick tour http://tess.elixir-uk.org/materials/biomodels-database-quick-tour BioModels Database: Quick tour from http://www.ebi.ac.uk/training/online/course/biomodels-database-quick-tour. Systems 2016-06-14
Biomedical data: Ethical, legal and social implications

Biomedical data: Ethical, legal and social implications from http://www.ebi.ac.uk/training/online/course/biomedical-data-ethical-legal-and-social-implicati.

Keywords: Cross domain

Biomedical data: Ethical, legal and social implications http://tess.elixir-uk.org/materials/biomedical-data-ethical-legal-and-social-implications Biomedical data: Ethical, legal and social implications from http://www.ebi.ac.uk/training/online/course/biomedical-data-ethical-legal-and-social-implicati. Cross domain 2016-06-14
Biomacromolecular structures: An introduction to EMBL-EBI resources

Biomacromolecular structures: An introduction to EMBL-EBI resources from http://www.ebi.ac.uk/training/online/course/biomacromolecular-structures-introduction-ebi-reso.

Keywords: Proteins

Biomacromolecular structures: An introduction to EMBL-EBI resources http://tess.elixir-uk.org/materials/biomacromolecular-structures-an-introduction-to-embl-ebi-resources Biomacromolecular structures: An introduction to EMBL-EBI resources from http://www.ebi.ac.uk/training/online/course/biomacromolecular-structures-introduction-ebi-reso. Proteins 2016-06-14
Biocuration: An introduction

Biocuration: An introduction from http://www.ebi.ac.uk/training/online/course/biocuration-introduction.

Keywords: Proteins, Cross domain

Biocuration: An introduction http://tess.elixir-uk.org/materials/biocuration-an-introduction Biocuration: An introduction from http://www.ebi.ac.uk/training/online/course/biocuration-introduction. Proteins, Cross domain 2016-06-14
ArrayExpress: Quick tour

This quick tour provides an overview of EMBL-EBI’s functional genomics database ArrayExpress. This course was updated in December 2015. An undergraduate-level understanding of biology is an advantage. You may wish to have a look at our Functional genomics: An introduction to EMBL-EBI...

Scientific topics: Gene expression, Gene expression and microarray, Functional genomics

Keywords: Gene Expression

ArrayExpress: Quick tour http://tess.elixir-uk.org/materials/arrayexpress-quick-tour This quick tour provides an overview of EMBL-EBI’s functional genomics database ArrayExpress. This course was updated in December 2015. An undergraduate-level understanding of biology is an advantage. You may wish to have a look at our Functional genomics: An introduction to EMBL-EBI resources before taking this course. Gene expression Gene expression and microarray Functional genomics Gene Expression Beginners 2016-06-14
Variant-calling

No description available

Keywords: Alignment, Annotation, BAM, BCF, De-novo-transcriptome-assembly, Exploratory-analysis, FASTQ, Pre-processing, QC, Statistical-model, Variant-calling, VCF

Variant-calling http://tess.elixir-uk.org/materials/variant-calling No description available Alignment, Annotation, BAM, BCF, De-novo-transcriptome-assembly, Exploratory-analysis, FASTQ, Pre-processing, QC, Statistical-model, Variant-calling, VCF
Guide to R swirl interactive lessons

This handout describes how to download the swirl package and start the basic interactive lessons to learn R.

Keywords: R-programming

Guide to R swirl interactive lessons http://tess.elixir-uk.org/materials/guide-to-r-swirl-interactive-lessons This handout describes how to download the swirl package and start the basic interactive lessons to learn R. R-programming
Day 4 - RNA-Seq Analysis

Day 4 focuses on the final steps after production of significant gene lists, including gene clustering, visualization, and annotation.

Keywords: Exploratory-analysis, Differential-expression, Statistical-model, Annotation

Day 4 - RNA-Seq Analysis http://tess.elixir-uk.org/materials/day-4-rna-seq-analysis Day 4 focuses on the final steps after production of significant gene lists, including gene clustering, visualization, and annotation. Exploratory-analysis, Differential-expression, Statistical-model, Annotation
Statistics and RNA-Seq

This lecture and optional practical introduce the relevant statistical concepts along with its computational counterparts used on a common RNA-Seq data analysis workflow.

Keywords: FASTQ, Alignment, BAM, Differential-expression, Statistical-model, Exploratory-analysis

Statistics and RNA-Seq http://tess.elixir-uk.org/materials/statistics-and-rna-seq This lecture and optional practical introduce the relevant statistical concepts along with its computational counterparts used on a common RNA-Seq data analysis workflow. FASTQ, Alignment, BAM, Differential-expression, Statistical-model, Exploratory-analysis
Introduction to RNA-seq analysis 2014

This lecture gives an overview how to perform an RNA-seq experiment. A general RNA-seq workflow is outlined when a good quality genome sequence is available for your species.

Keywords: Alignment, Differential-expression, Feature-summarisation, Pre-processing, QC

Introduction to RNA-seq analysis 2014 http://tess.elixir-uk.org/materials/introduction-to-rna-seq-analysis-2014 This lecture gives an overview how to perform an RNA-seq experiment. A general RNA-seq workflow is outlined when a good quality genome sequence is available for your species. Alignment, Differential-expression, Feature-summarisation, Pre-processing, QC
Introduction to R practice codes

These codes give an overview of R object types and programming language structure.

Keywords: R-programming

Introduction to R practice codes http://tess.elixir-uk.org/materials/introduction-to-r-practice-codes These codes give an overview of R object types and programming language structure. R-programming
RNA-seq training PSB 2013

This lecture gives an overview how to perform an RNA-seq experiment. First, the Illumina sequencing platform is briefly covered, followed by the different file formats in NGS. Next, an RNA-seq workflow is outlined, starting from the raw data up to differential expression. The recommended coverage...

Keywords: Alignment, Differential-expression, Feature-summarisation, Pre-processing, QC

RNA-seq training PSB 2013 http://tess.elixir-uk.org/materials/rna-seq-training-psb-2013 This lecture gives an overview how to perform an RNA-seq experiment. First, the Illumina sequencing platform is briefly covered, followed by the different file formats in NGS. Next, an RNA-seq workflow is outlined, starting from the raw data up to differential expression. The recommended coverage and sequencing mode is touched upon as well as the effect on your analysis of divergence of your genotype compared to the reference. Alignment, Differential-expression, Feature-summarisation, Pre-processing, QC
Material for the course RNA-seq data analysis with Chipster

This material covers the whole RNA-seq data analysis pipeline, from quality control of raw reads to differential expression analysis.

Scientific topics: RNA-Seq

Keywords: RNA-Seq, FASTQ, QC, Pre-processing, Alignment, BAM, Expression-estimation, Feature-summarisation, Differential-expression, Statistical-model, Exploratory-analysis

Material for the course RNA-seq data analysis with Chipster http://tess.elixir-uk.org/materials/material-for-the-course-rna-seq-data-analysis-with-chipster This material covers the whole RNA-seq data analysis pipeline, from quality control of raw reads to differential expression analysis. RNA-Seq RNA-Seq, FASTQ, QC, Pre-processing, Alignment, BAM, Expression-estimation, Feature-summarisation, Differential-expression, Statistical-model, Exploratory-analysis
RNA-seq module Bert Overduin

All material concerning RNA-seq analysis

Scientific topics: RNA-Seq

Keywords: RNA-Seq, Pre-processing, QC, Alignment

RNA-seq module Bert Overduin http://tess.elixir-uk.org/materials/rna-seq-module-bert-overduin All material concerning RNA-seq analysis RNA-Seq RNA-Seq, Pre-processing, QC, Alignment
Nicolas Delhomme and Bastian Schiffthaler

This merely lists the various courses at which we taught RNA-Seq data

Scientific topics: RNA-Seq

Keywords: FASTQ, GFF3, BAM, Populus-tremula, RNA-Seq, Pre-processing, QC, Alignment, Annotation, Expression-estimation, Differential-expression, R-programming

Nicolas Delhomme and Bastian Schiffthaler http://tess.elixir-uk.org/materials/nicolas-delhomme-and-bastian-schiffthaler This merely lists the various courses at which we taught RNA-Seq data RNA-Seq FASTQ, GFF3, BAM, Populus-tremula, RNA-Seq, Pre-processing, QC, Alignment, Annotation, Expression-estimation, Differential-expression, R-programming
Expression estimation

This introduces how to summarise short read alignments by the annotation of interest to obtain a count-table; i.e. the structure necessary to most downstream expression based analyses. Here, the focus is put on gene-expression, but the aspects of transcript-expression are briefly addressed.

Scientific topics: RNA-Seq

Keywords: GFF3, BAM, Populus-tremula, RNA-Seq, Expression-estimation

Expression estimation http://tess.elixir-uk.org/materials/expression-estimation This introduces how to summarise short read alignments by the annotation of interest to obtain a count-table; i.e. the structure necessary to most downstream expression based analyses. Here, the focus is put on gene-expression, but the aspects of transcript-expression are briefly addressed. RNA-Seq GFF3, BAM, Populus-tremula, RNA-Seq, Expression-estimation
Example “Variant Calling”

No description available

Example “Variant Calling” http://tess.elixir-uk.org/materials/example-variant-calling No description available
Mapping Quality Control

Perform QC on aligned data

Keywords: Alignment, BAM, QC

Mapping Quality Control http://tess.elixir-uk.org/materials/mapping-quality-control Perform QC on aligned data Alignment, BAM, QC
Day 3 - RNA-Seq Analysis

Day 3 focuses on statistical analysis of RNA-Seq data and identification of differentiall expressed genes in multiple comparisons.

Keywords: QC, Exploratory-analysis, Differential-expression, Statistical-model, Pre-processing

Day 3 - RNA-Seq Analysis http://tess.elixir-uk.org/materials/day-3-rna-seq-analysis Day 3 focuses on statistical analysis of RNA-Seq data and identification of differentiall expressed genes in multiple comparisons. QC, Exploratory-analysis, Differential-expression, Statistical-model, Pre-processing
RNA-Seq

RNA-Seq is an high throughput sequencing method commonly used to assess gene expression.

RNA-Seq http://tess.elixir-uk.org/materials/keywords RNA-Seq is an high throughput sequencing method commonly used to assess gene expression.
ChIP-Seq

No description available

Keywords: ChIP-Seq, Experimental-design, QC, ChIP-Seq-QC, Data-format, Alignment, Peak-calling, Differential-binding, Annotation

ChIP-Seq http://tess.elixir-uk.org/materials/chip-seq No description available ChIP-Seq, Experimental-design, QC, ChIP-Seq-QC, Data-format, Alignment, Peak-calling, Differential-binding, Annotation
Tutorial

This file describes the main tutorial PDF file. Almost all tutorials and hands-on practices are indeed collated in a single document. In addition to this PDF, R code excerpts and installation instructions are also provided.

Scientific topics: RNA-Seq

Keywords: FASTQ, GFF3, BAM, Populus-tremula, RNA-Seq, Pre-processing, QC, Alignment, Annotation, Expression-estimation, R-programming

Tutorial http://tess.elixir-uk.org/materials/tutorial This file describes the main tutorial PDF file. Almost all tutorials and hands-on practices are indeed collated in a single document. In addition to this PDF, R code excerpts and installation instructions are also provided. RNA-Seq FASTQ, GFF3, BAM, Populus-tremula, RNA-Seq, Pre-processing, QC, Alignment, Annotation, Expression-estimation, R-programming
Prerequisite

No description available

Scientific topics: Statistics and probability

Keywords: Unix, Linux, R-programming, Statistics

Prerequisite http://tess.elixir-uk.org/materials/prerequisite No description available Statistics and probability Unix, Linux, R-programming, Statistics
Preprocessing RNA-Seq data

Quality assessment and preprocessing of RNA-seq data. Description of FastQ format

Keywords: Pre-processing

Preprocessing RNA-Seq data http://tess.elixir-uk.org/materials/preprocessing Quality assessment and preprocessing of RNA-seq data. Description of FastQ format Pre-processing
RNA-Seq Analysis with Biocluster and R

Sequencing of RNA (RNA-Seq) is the latest method to assess global gene expression because it

Scientific topics: RNA-Seq

Keywords: RNA-Seq, Alignment, Annotation, BAM, Differential-expression, Exploratory-analysis, Expression-estimation, FASTA, FASTQ, Feature-summarisation, Pre-processing, QC, Statistical-model

RNA-Seq Analysis with Biocluster and R http://tess.elixir-uk.org/materials/rna-seq-analysis-with-biocluster-and-r Sequencing of RNA (RNA-Seq) is the latest method to assess global gene expression because it RNA-Seq RNA-Seq, Alignment, Annotation, BAM, Differential-expression, Exploratory-analysis, Expression-estimation, FASTA, FASTQ, Feature-summarisation, Pre-processing, QC, Statistical-model
Material provided by Charlotte Soneson

This folder contains material provided by Charlotte Soneson. The following material is included:

Scientific topics: RNA-Seq

Keywords: RNA-Seq, Differential-expression, Statistical-model, Exploratory-analysis

Material provided by Charlotte Soneson http://tess.elixir-uk.org/materials/material-provided-by-charlotte-soneson This folder contains material provided by Charlotte Soneson. The following material is included: RNA-Seq RNA-Seq, Differential-expression, Statistical-model, Exploratory-analysis
Differential expression analysis

This lecture covers the process from count matrix to statistical analysis results (differential expression). More specifically, it covers experimental design, normalization, statistical modeling and parameter estimation, multiple hypothesis testing and a more detailed look at some of the most...

Keywords: Differential-expression, Statistical-model

Differential expression analysis http://tess.elixir-uk.org/materials/differential-expression-analysis This lecture covers the process from count matrix to statistical analysis results (differential expression). More specifically, it covers experimental design, normalization, statistical modeling and parameter estimation, multiple hypothesis testing and a more detailed look at some of the most common differential expression methods as well as a comparison between them. Differential-expression, Statistical-model
RNA-seq module Eija Korpelainen

All material concerning RNA-seq data analysis with Chipster

Keywords: FASTQ, QC, Pre-processing, Alignment, BAM, Expression-estimation, Feature-summarisation, Differential-expression, Statistical-model, Exploratory-analysis

RNA-seq module Eija Korpelainen http://tess.elixir-uk.org/materials/rna-seq-module-eija-korpelainen All material concerning RNA-seq data analysis with Chipster FASTQ, QC, Pre-processing, Alignment, BAM, Expression-estimation, Feature-summarisation, Differential-expression, Statistical-model, Exploratory-analysis
Populus tremula shows no evidence of sexual dimorphism

**Background:** Although the majority of plant species are co-sexual, being either monoecious or hermaphroditic, a significant number are dioecious, having separate male and female individuals. Evolutionary theory suggests that males and females may develop sexually dimorphic phenotypic and...

Scientific topics: RNA-Seq

Keywords: FASTQ, GFF3, BAM, Populus-tremula, RNA-Seq, Pre-processing, QC, Alignment, Annotation, Expression-estimation, Differential-expression

Populus tremula shows no evidence of sexual dimorphism http://tess.elixir-uk.org/materials/populus-tremula-shows-no-evidence-of-sexual-dimorphism **Background:** Although the majority of plant species are co-sexual, being either monoecious or hermaphroditic, a significant number are dioecious, having separate male and female individuals. Evolutionary theory suggests that males and females may develop sexually dimorphic phenotypic and biochemical traits concordant with each sex having different optimal strategies of resource investment to maximise reproductive success and fitness. The establishment of such sexual dimorphism would result in changes in gene expression patterns in non-floral organs. RNA-Seq FASTQ, GFF3, BAM, Populus-tremula, RNA-Seq, Pre-processing, QC, Alignment, Annotation, Expression-estimation, Differential-expression
Nicolas Delhomme - Bastian Schiffthaler - October 2014 EMBO course material

Material for the course held on EBI Campus, Welcome Trust Center, Hinxton, UK on 20-26th, October 2014. The material cover general RNA-Seq data pre-processing as described in these [guidelines](http://www.epigenesys.eu/en/protocols/bio-informatics/1283-guidelines-for-rna-seq-data-analysis) and...

Scientific topics: RNA-Seq

Keywords: FASTQ, GFF3, BAM, Populus-tremula, RNA-Seq, Pre-processing, QC, Alignment, Annotation, Expression-estimation, Differential-expression, R-programming

Nicolas Delhomme - Bastian Schiffthaler - October 2014 EMBO course material http://tess.elixir-uk.org/materials/nicolas-delhomme-bastian-schiffthaler-october-2014-embo-course-material Material for the course held on EBI Campus, Welcome Trust Center, Hinxton, UK on 20-26th, October 2014. The material cover general RNA-Seq data pre-processing as described in these [guidelines](http://www.epigenesys.eu/en/protocols/bio-informatics/1283-guidelines-for-rna-seq-data-analysis) and reproduces the Differential Expression analysis conducted in Robinson, Delhomme et al., 2014. RNA-Seq FASTQ, GFF3, BAM, Populus-tremula, RNA-Seq, Pre-processing, QC, Alignment, Annotation, Expression-estimation, Differential-expression, R-programming