Quality Control in Sequencing Experiments
This course looks at the different ways in which sequencing based studies can fail and the options for visualisation and QC which allow you to identify and diagnose these failures at an early stage. It is designed to be of use to anyone who is using sequencing as part of their research, not just...
Scientific topics: Quality affairs
Keywords: Babraham Institute
Quality Control in Sequencing Experiments
http://www.bioinformatics.babraham.ac.uk/training.html#sequenceqc
http://tess.elixir-uk.org/materials/quality-control-in-sequencing-experiments
This course looks at the different ways in which sequencing based studies can fail and the options for visualisation and QC which allow you to identify and diagnose these failures at an early stage. It is designed to be of use to anyone who is using sequencing as part of their research, not just those who are running sequencing facilities.
Simon Andrews
Laura Biggins
Boo Virk
Quality affairs
Babraham Institute
Extracting biological information from gene lists
Many experimental designs end up producing lists of hits, usually based around genes or transcripts. Sometimes these lists are small enough that they can be examined individually, but often it is useful to do a more structured functional analysis to try to automatically determine any interesting...
Keywords: Functional analysis, Gene lists, Babraham Institute
Extracting biological information from gene lists
http://www.bioinformatics.babraham.ac.uk/training.html#fagl
http://tess.elixir-uk.org/materials/extracting-biological-information-from-gene-lists
Many experimental designs end up producing lists of hits, usually based around genes or transcripts. Sometimes these lists are small enough that they can be examined individually, but often it is useful to do a more structured functional analysis to try to automatically determine any interesting biological themes which turn up in the lists.
This course looks at the various software packages, databases and statistical methods which may be of use in performing such an analysis. As well as being a practical guide to performing these types of analysis the course will also look at the types of artefacts and bias which can lead to false conclusions about functionality and will look at the appropriate ways to both run the analysis and present the results for publication.
Simon Andrews
Laura Biggins
Functional analysis, Gene lists, Babraham Institute
Sample size estimation
Power analysis is a method to estimate the samples sizes needed to detect statistical effects with high probability. This course provides an introduction to the basic principles of power analysis and goes through the variables for power calculation. It also addresses the issues associated with...
Keywords: Power analysis, Sample size, Babraham Institute
Sample size estimation
http://www.bioinformatics.babraham.ac.uk/training.html#power
http://tess.elixir-uk.org/materials/sample-size-estimation
Power analysis is a method to estimate the samples sizes needed to detect statistical effects with high probability. This course provides an introduction to the basic principles of power analysis and goes through the variables for power calculation. It also addresses the issues associated with technical and biological replicates. The program is intended for people who have no prior knowledge of power. Basic statistical knowledge is extremely useful but prior attendance on a statistics course is not compulsary.
Anne Segonds-Pichon
Power analysis, Sample size, Babraham Institute
Statistical Analysis using SPSS
Statistics are an important part of most modern studies and being able to effectively use a statistics package can help you to understand your results. This course provides an introduction to statistics illustrated though the use of the friendly SPSS package.
Scientific topics: Statistics and probability
Keywords: Babraham Institute
Statistical Analysis using SPSS
http://www.bioinformatics.babraham.ac.uk/training.html#spss
http://tess.elixir-uk.org/materials/statistical-analysis-using-spss
Statistics are an important part of most modern studies and being able to effectively use a statistics package can help you to understand your results. This course provides an introduction to statistics illustrated though the use of the friendly SPSS package.
Anne Segonds-Pichon
Statistics and probability
Babraham Institute
Statistical Analysis using GraphPad Prism
GraphPad Prism is a powerful and friendly package which allows you to plot and analyse your data. This course acts not only as an introduction to Prism, but also goes through the basic statistical knowledge which should allow you to make the most of your data.
Keywords: GraphPad Prism, Babraham Institute
Statistical Analysis using GraphPad Prism
http://www.bioinformatics.babraham.ac.uk/training.html#prism
http://tess.elixir-uk.org/materials/statistical-analysis-using-graphpad-prism
GraphPad Prism is a powerful and friendly package which allows you to plot and analyse your data. This course acts not only as an introduction to Prism, but also goes through the basic statistical knowledge which should allow you to make the most of your data.
Anne Segonds-Pichon
GraphPad Prism, Babraham Institute
Scientific Figure Design
This course provides a practical guide to producing figures for use in reports and publications. It is a wide ranging course which looks at how to design figures to clearly and fairly represent your data, the practical aspects of graph creation, the allowable manipulation of bitmap images and...
Scientific topics: Data visualisation
Keywords: Figure design, Babraham Institute
Scientific Figure Design
http://www.bioinformatics.babraham.ac.uk/training.html#figuredesign
http://tess.elixir-uk.org/materials/scientific-figure-design
This course provides a practical guide to producing figures for use in reports and publications. It is a wide ranging course which looks at how to design figures to clearly and fairly represent your data, the practical aspects of graph creation, the allowable manipulation of bitmap images and compositing and editing of final figures.
The course will use a number of different open source software packages and is illustrated with a number of example figures adapted from common analysis tools.
Simon Andrews
Anne Segonds-Pichon
Boo Virk
Data visualisation
Figure design, Babraham Institute
An Introduction to Unix
Increasing amounts of bioinformatics work is done in a command line unix environment. Most large scale processing applications are written for unix and most large scale compute environments are also based on this.
This course provides an introduction to the concepts of unix and provides a...
Keywords: Unix, Babraham Institute
An Introduction to Unix
http://www.bioinformatics.babraham.ac.uk/training.html#unix
http://tess.elixir-uk.org/materials/an-introduction-to-unix
Increasing amounts of bioinformatics work is done in a command line unix environment. Most large scale processing applications are written for unix and most large scale compute environments are also based on this.
This course provides an introduction to the concepts of unix and provides a practical introduction to working in this environment. Internally we link this course to a more specific course illustrating the use of our internal cluster environment and this part of the course could be adapted for other sites with different compute infrastructure.
Simon Andrews
Unix, Babraham Institute
An Introduction to ggplot
Ggplot is the most popular plotting extension to R and replicates many of the graph types found in the core plotting libraries. This course provides an introduction to the ggplot2 libraries and gives a practical guide for how to use these to create different types of graphs.
Keywords: ggPlot, Babraham Institute
An Introduction to ggplot
http://www.bioinformatics.babraham.ac.uk/training.html#ggplot
http://tess.elixir-uk.org/materials/an-introduction-to-ggplot
Ggplot is the most popular plotting extension to R and replicates many of the graph types found in the core plotting libraries. This course provides an introduction to the ggplot2 libraries and gives a practical guide for how to use these to create different types of graphs.
Anne Segonds-Pichon
ggPlot, Babraham Institute
Plotting complex figures with R
This course is a comprehensive guide to the use of the built in R plotting functionality to construct everything from customised simple plots to complex multi-layered figures. It follows on from the material in our introductory R course and participants are expected to have a basic understanding...
Keywords: Graph plotting, R Programming, Babraham Institute
Plotting complex figures with R
http://www.bioinformatics.babraham.ac.uk/training.html#rplotting
http://tess.elixir-uk.org/materials/plotting-complex-figures-with-r
This course is a comprehensive guide to the use of the built in R plotting functionality to construct everything from customised simple plots to complex multi-layered figures. It follows on from the material in our introductory R course and participants are expected to have a basic understanding of R - enough to load and do basic manipulation of datasets.
Simon Andrews
Graph plotting, R Programming, Babraham Institute
Advanced R
This course follows on from the introductory course. It goes into more detail on practical guides to filtering and combining complex data sets. It also looks at other core R concepts such as looping with apply statements and using packages. Finally it looks at how to document your R analyses and...
Keywords: R programming, Babraham Institute
Advanced R
http://www.bioinformatics.babraham.ac.uk/training.html#radvanced
http://tess.elixir-uk.org/materials/advanced-r
This course follows on from the introductory course. It goes into more detail on practical guides to filtering and combining complex data sets. It also looks at other core R concepts such as looping with apply statements and using packages. Finally it looks at how to document your R analyses and generate complete analysis reports.
Simon Andrews
R programming, Babraham Institute
Introduction to R
R is a popular language and environment that allows powerful and fast manipulation of data, offering many statistical and graphical options. This course aims to introduce R as a tool for statistics and graphics, with the main aim being to become comfortable with the R environment. It will focus...
Scientific topics: Software engineering
Keywords: R programming, Babraham Institute
Introduction to R
http://www.bioinformatics.babraham.ac.uk/training.html#rintro
http://tess.elixir-uk.org/materials/introduction-to-r
R is a popular language and environment that allows powerful and fast manipulation of data, offering many statistical and graphical options. This course aims to introduce R as a tool for statistics and graphics, with the main aim being to become comfortable with the R environment. It will focus on entering and manipulating data in R and producing simple graphs. A few functions for basic statistics will be briefly introduced, but statistical functions will not be covered in detail.
Laura Biggins
Simon Andrews
Software engineering
R programming, Babraham Institute
Learning to Program with Perl
This course aims to introduce the basic features of the Perl language. At the end you should have everything you need to write moderately complicated programs, and enough pointers to other resources to get you started on bigger projects. The course tries to provide a grounding in the basic theory...
Keywords: perl, Babraham Institute
Learning to Program with Perl
http://www.bioinformatics.babraham.ac.uk/training.html#perl
http://tess.elixir-uk.org/materials/learning-to-program-with-perl
This course aims to introduce the basic features of the Perl language. At the end you should have everything you need to write moderately complicated programs, and enough pointers to other resources to get you started on bigger projects. The course tries to provide a grounding in the basic theory you'll need to write programs in any language as well as an appreciation for the right way to do things in Perl.
Simon Andrews
perl, Babraham Institute
Analysing Mapped Sequence Data with SeqMonk
SeqMonk is a program which can analyse large data sets of mapped genomic positions. It is most commonly used to work with data coming from high-throughput sequencing pipelines.
The program allows you to view your reads against an annotated genome and to quantitate and filter your data to let...
Scientific topics: Sequence alignment
Keywords: Babraham Institute
Analysing Mapped Sequence Data with SeqMonk
http://www.bioinformatics.babraham.ac.uk/training.html#seqmonk
http://tess.elixir-uk.org/materials/analysing-mapped-sequence-data-with-seqmonk
SeqMonk is a program which can analyse large data sets of mapped genomic positions. It is most commonly used to work with data coming from high-throughput sequencing pipelines.
The program allows you to view your reads against an annotated genome and to quantitate and filter your data to let you identify regions of interest. It is a friendly way to explore and analysis very large datasets.
This course provides an introduction to the main features of SeqMonk and will run through the anlaysis of a couple of different datasets to show what sort of analysis options it provides.
Simon Andrews
Sequence alignment
Babraham Institute