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Keywords: NGS 

Chip-seq: Motif Analysis Tutorial

Introduction Goal The aim is to : Get familiar with motif analysis of ChIP-seq data. Learn de novo motif discovery methods. In practice : Motif discovery with peak-motifs Differential analysis Random controls

Keywords: Chip-seq, Motif analysis, NGS, Pattern recognition

Chip-seq: Motif Analysis Tutorial http://tess.elixir-uk.org/materials/chip-seq-motif-analysis-tutorial Introduction Goal The aim is to : Get familiar with motif analysis of ChIP-seq data. Learn de novo motif discovery methods. In practice : Motif discovery with peak-motifs Differential analysis Random controls Chip-seq, Motif analysis, NGS, Pattern recognition
Chip-seq: Functional Annotation tutorial

Global Objective Given a set of ChIP-seq peaks annotate them in order to find associated genes, genomic categories and functional terms.

Keywords: Chip-seq, Functional Annotation, NGS

Chip-seq: Functional Annotation tutorial http://tess.elixir-uk.org/materials/chip-seq-functional-annotation-tutorial Global Objective Given a set of ChIP-seq peaks annotate them in order to find associated genes, genomic categories and functional terms. Chip-seq, Functional Annotation, NGS
Docker version of Welsh Gene Park NGS course

Docker version of Welsh Gene Park NGS course

Keywords: NGS, IFR, Institute of Food Research, Quadram Institute

Docker version of Welsh Gene Park NGS course http://tess.elixir-uk.org/materials/docker-version-of-welsh-gene-park-ngs-course Docker version of Welsh Gene Park NGS course NGS, IFR, Institute of Food Research, Quadram Institute
IFR Dockerised version of the Welsh Genepark's Introduction to Command-line NGS Analysis

The IFR Dockerised version of the Welsh Genepark's Introduction to Command-line NGS Analysis course

Keywords: NGS, Command line, IFR, Institute of Food Research, Quadram Institute

IFR Dockerised version of the Welsh Genepark's Introduction to Command-line NGS Analysis http://tess.elixir-uk.org/materials/ifr-dockerised-version-of-the-welsh-genepark-s-introduction-to-command-line-ngs-analysis The IFR Dockerised version of the Welsh Genepark's Introduction to Command-line NGS Analysis course NGS, Command line, IFR, Institute of Food Research, Quadram Institute
Variant Filtering

Use cases: Extact a subset of variants Combine variants from several analysis Compare obtained variants from several data types Identify new variants compare to a reference list Apply specific filters for Chip Design

Keywords: NGS, Variant calling

Variant Filtering http://tess.elixir-uk.org/materials/variant-filtering-98171288-9eb3-49d3-b627-8ae1ead26885 Use cases: Extact a subset of variants Combine variants from several analysis Compare obtained variants from several data types Identify new variants compare to a reference list Apply specific filters for Chip Design NGS, Variant calling
Chip-seq: Pattern Analysis tutorial

Goal The aim is to : Get familiar with motif analysis of ChIP-seq data. Learn de novo motif discovery methods. In practice : Motif discovery with peak-motifs Differential analysis Random controls

Keywords: Chip-seq, NGS, Pattern recognition

Chip-seq: Pattern Analysis tutorial http://tess.elixir-uk.org/materials/chip-seq-pattern-analysis-tutorial Goal The aim is to : Get familiar with motif analysis of ChIP-seq data. Learn de novo motif discovery methods. In practice : Motif discovery with peak-motifs Differential analysis Random controls Chip-seq, NGS, Pattern recognition
Chip-seq: Peak calling tutorial

The aim is to : Understand how to process reads to obtain peaks (peak-calling). Become familiar with differential analysis of peaks In practice : Obtain dataset from GEO Analyze mapped reads Obtain set(s) of peaks, handle replicates Differential analysis of peak

Keywords: Chip-seq, NGS, Peak calling

Chip-seq: Peak calling tutorial http://tess.elixir-uk.org/materials/chip-seq-peak-calling-tutorial The aim is to : Understand how to process reads to obtain peaks (peak-calling). Become familiar with differential analysis of peaks In practice : Obtain dataset from GEO Analyze mapped reads Obtain set(s) of peaks, handle replicates Differential analysis of peak Chip-seq, NGS, Peak calling
NGS data exploration with the MicroScope Platform

Exploring data annotation on the genomics and transcriptomics levels with the MicroScope Platform and its tools

Keywords: Genomics, NGS, RNA-seq, SNP, Transcriptomics

NGS data exploration with the MicroScope Platform http://tess.elixir-uk.org/materials/ngs-data-exploration-with-the-microscope-platform Exploring data annotation on the genomics and transcriptomics levels with the MicroScope Platform and its tools Genomics, NGS, RNA-seq, SNP, Transcriptomics
Chip-seq: Discovering motifs in peaks with RSAT

Read mapping: from raw reads to aligned reads. Peak calling: from aligned reads to regions/peaks of high read density. ChIP-seq annotation Identification of genes related to the peaks. Profiles of ChIP-seq reads around reference points (TSS, histone marks,). Functional enrichment of the...

Keywords: Chip Seq, Motif Analysis, NGS

Chip-seq: Discovering motifs in peaks with RSAT http://tess.elixir-uk.org/materials/chip-seq-discovering-motifs-in-peaks-with-rsat Read mapping: from raw reads to aligned reads. Peak calling: from aligned reads to regions/peaks of high read density. ChIP-seq annotation Identification of genes related to the peaks. Profiles of ChIP-seq reads around reference points (TSS, histone marks,). Functional enrichment of the genes related to the peaks. Chip Seq, Motif Analysis, NGS
Chip Seq: Annotation and visualization Lesson

How to add biological meaning to peaks

Keywords: Annotation, Chip-seq, Data Visualization, NGS

Chip Seq: Annotation and visualization Lesson http://tess.elixir-uk.org/materials/chip-seq-annotation-and-visualization-lesson How to add biological meaning to peaks Annotation, Chip-seq, Data Visualization, NGS
Chip Seq: Annotation and visualization Tutorial

Global Objective Given a set of ChIP-seq peaks annotate them in order to find associated genes, genomic categories and functional terms.

Keywords: Annotation, Chip-seq, Data Visualization, NGS

Chip Seq: Annotation and visualization Tutorial http://tess.elixir-uk.org/materials/chip-seq-annotation-and-visualization-tutorial Global Objective Given a set of ChIP-seq peaks annotate them in order to find associated genes, genomic categories and functional terms. Annotation, Chip-seq, Data Visualization, NGS
Visualization of NGS data with IGV

Visualisation of next-gen sequencing data with Integrative Genomics Viewer

Keywords: Data visualization, Genomics, NGS

Visualization of NGS data with IGV http://tess.elixir-uk.org/materials/visualization-of-ngs-data-with-igv Visualisation of next-gen sequencing data with Integrative Genomics Viewer Data visualization, Genomics, NGS
RADSeq Data Analysis

Introduction to RADSeq through STACKS on Galaxy

Keywords: NGS

RADSeq Data Analysis http://tess.elixir-uk.org/materials/radseq-data-analysis Introduction to RADSeq through STACKS on Galaxy NGS
3-day hands-on NGS workshop

This document is the trainee exercise booklet for a 3 day hands-on Next Generation Sequencing workshop developed by Bioplatforms Australia, CSIRO and EMBL-EBI.  This workshop is aimed at bench biologists, PhD students or early career postdoctoral researchers or those new to the analysis of NGS...

Keywords: NGS, ChIPSeq, De novo genome assembly, RNASeq

3-day hands-on NGS workshop http://tess.elixir-uk.org/materials/3-day-hands-on-ngs-workshop This document is the trainee exercise booklet for a 3 day hands-on Next Generation Sequencing workshop developed by Bioplatforms Australia, CSIRO and EMBL-EBI.  This workshop is aimed at bench biologists, PhD students or early career postdoctoral researchers or those new to the analysis of NGS data and assumes no prior knowledge of bioinformatics. This course covers the following topics: data quality control, read alignment, ChIPSeq, RNASeq and de novo assembly. The workshop starts with the Intro to the shell material from Software Carpentry (http://software-carpentry.org/v4/shell/index.html).  Introductory lectures on each topic are presented before trainees move on that topic. The workshop has been delivered a number of times in different cities in Australia to date, in a roadshow style, and is designed to run on virtual machines on the Australian research cloud. Trainees then use NoMachine NX client to provide a remote desktop like connection to their own dedicated VM for the duration of of the workshop.  Various contributors made this workshop possible and they are credited in the introduction to each section. NGS, ChIPSeq, De novo genome assembly, RNASeq Bench biologists Life Science Researchers PhD students postdocs 2014-01-17 2017-10-09