Register training material
16 materials found

Authors: allegra.via Via  or stortebecker 


Proteomics - Peptide and Protein ID using OpenMS tools

Training material for proteomics workflows in Galaxy

Questions of the tutorial:

  • How to convert LC-MS/MS raw files?
  • How to identify peptides?
  • How to identify proteins?
  • How to evaluate the results?

Objectives of the tutorial:

  • Protein identification from LC-MS/MS raw files.

Resource type: Tutorial

Proteomics - Detection and quantitation of N-termini (degradomics) via N-TAILS

Training material for proteomics workflows in Galaxy

Questions of the tutorial:

  • How can protein N-termini be enriched for LC-MS/MS?
  • How to analyze the LC-MS/MS data?

Objectives of the tutorial:

  • Run an N-TAILS data analysis.

Resource type: Tutorial

Proteomics - Peptide and Protein Quantification via Stable Isotope Labelling (SIL)

Training material for proteomics workflows in Galaxy

Questions of the tutorial:

  • What are MS1 features?
  • How to quantify based on MS1 features?
  • How to map MS1 features to MS2 identifications?
  • How to evaluate and optimize the results?

Objectives of the tutorial:

  • MS1 feature...

Resource type: Tutorial

Proteomics - Peptide and Protein ID using SearchGUI and PeptideShaker

Training material for proteomics workflows in Galaxy

Questions of the tutorial:

  • How to convert LC-MS/MS raw files?
  • How to identify peptides?
  • How to identify proteins?
  • How to evaluate the results?

Objectives of the tutorial:

  • Protein identification from LC-MS/MS raw files.

Resource type: Tutorial

Proteomics - Secretome Prediction

Training material for proteomics workflows in Galaxy

Questions of the tutorial:

  • How to predict cellular protein localization based upon GO-terms?
  • How to combine multiple localization predictions?

Objectives of the tutorial:

  • Predict proteins in the cellular secretome by using...

Resource type: Tutorial

Proteomics - Label-free versus Labelled - How to Choose Your Quantitation Method

Training material for proteomics workflows in Galaxy

Questions of the tutorial:

  • What are benefits and drawbacks of different quantitation methods?
  • How to choose which quantitation method bests suits my need?

Objectives of the tutorial:

  • Choose your quantitation method.

Resource type: Tutorial

Proteomics - Protein FASTA Database Handling

Training material for proteomics workflows in Galaxy

Questions of the tutorial:

  • How to download protein FASTA databases of a certain organism?
  • How to download a contaminant database?
  • How to create a decoy database?
  • How to combine databases?

Objectives of the tutorial:

  • Creation of a...

Resource type: Tutorial

Introduction to Biopython

This is a module from the "Python for Biologists" course. The module presents an introduction to Biopython. It shows how to deal with sequences and sequence records, how to download records from NCBI databases, how to run Blast and how to parse XML Blast outputs.

Keywords: Bioinformatics, Biopython, Programming, Python, Python biologists

Using R with Python

This is a module from the "Python for Biologists" course. It describes the Python module interfacing the R package for statistics. The module shows how to calculate mean, standard deviation, z-score and p-value of a set of numbers, and how to generate plots. Input files for the scripts presented...

Keywords: Programming, Python, Python biologists

Searching data using Python

This is a module from the "Python for Biologists" course. It describes how to use Python dictionary and set data structures to search your data. In particular, how to use a dictionary to represent the genetic code table and use it to translate a nucleotide sequence into a protein sequence, and...

Keywords: Programming, Python, Python biologists

Pattern Matching

This is a module from the "Python for Biologists" course. It teaches how to do pattern matching in Python, i.e. how to find a substring (or a set of substrings) in a string. To this aim, it introduces the regular expression syntax, and the tools needed to search regular expressions in biological...

Keywords: Pattern matching, Programming, Python, Python biologists

Writing functions in Python programming

This is a module from the "Python for Biologists" course. It deals with functions and how to write and use them. It also introduces namespaces and the tuple data structure. The module contains several exercises and suggested solutions. The text of exercises is also provided in a separate file. 

Scientific topics: Bioinformatics

Keywords: Programming, Python, Python biologists

Python Programs

This is a module from the "Python for Biologists" course. It deals with Python programs, how to write and run them, and how to provide input and generate output. The module also contains exercises and suggested solutions. 

Keywords: Programming, Python, Python biologists

Linear Motifs and Phosphorylation Sites

This is a lecture on linear motifs and phophorylation sites (P-sites). Some materials from other lectures are reused.
The lecture is basically about computational approaches to encode, predict, analyse, and use functional motifs and P-sites.
Here you can find:

    A definition of linear motifs and...

Keywords: Bioinformatics

Parsing data records using Python programming

This is a module from the "Python for Biologists" course. One typical problem in bioinformatics is parsing data files. This module explains how to parse FASTA files and GenBank records. It also introduces the if/elif/else construct to make choice in programming and the list  data structure. The...

Keywords: Bioinformatics, Programming, Python, Python biologists, Record parsing

Introduction to Unix

Introductory lecture to the Unix/Linux command-line

    Description of the computer shell and the command-line interface

    Differences between graphical and command-line interfaces

    The most commond Unix/Linux commands are provided

 
 

Keywords: Bioinformatics, Programming, Unixlinux