Authors: Janick Mathys or Simon Gladman
The questions this addresses are:
- How do we detect differences between a set of reads from M. tuberculosis (Mtb) and the Mtb reference genome
\nThe objectives are:
- How should we filter those variants
- How can we predict drug resistance from those variants
- How do we annotate those...
The questions this addresses are:
- How do we detect differences between a set of reads from a microorganism and a reference genome
\nThe objectives are:
- Find variants between a reference genome and a set of reads
- Visualise the SNP in context of the reads aligned to the genome
-...
The questions this addresses are:
- How can we annotate a bacterial genome?
- How can we visualize annotated genomic features?
\nThe objectives are:
- Load genome into Galaxy
- Annotate genome with Prokka
- View annotations in JBrowse
The questions this addresses are:
- I have short reads and long reads. How do I assemble a genome?
\nThe objectives are:
- Perform Quality Control on your reads
- Perform a Small genome Assembly with Unicycler
- Evaluate the Quality of the Assembly with Quast
- Annotate the assembly with...
The questions this addresses are:
- What are the factors that affect genome assembly?
- How does Genome assembly work?
\nThe objectives are:
- Perform an optimised Velvet assembly with the Velvet Optimiser
- Compare this assembly with those we did in the basic tutorial
- Perform an...
The objectives are:
- Have an understanding of what CVMFS is and how it works
- Install and configure the CVMFS client on a linux machine and mount the Galaxy reference data repository
- Configure your Galaxy to use these reference genomes and indices
The questions this addresses are:
- How can I find which version of Galaxy I'm running?
- How do I upgrade the Galaxy server to the latest version?
\nThe objectives are:
- Understand how to find the current Galaxy server version you are using
- How to find the latest release version
- How...
The objectives are:
- Have an understanding of what CVMFS is and how it works
- Install and configure the CVMFS client on a linux machine and mount the Galaxy reference data repository
- Configure your Galaxy to use these reference genomes and indices
- Use an Ansible playbook for all of the...
The questions this addresses are:
- How does pulsar work?
- How can I deploy it?
\nThe objectives are:
- Have an understanding of what Pulsar is and how it works
- Install and configure a RabbitMQ message queueing server
- Install and configure a Pulsar server on a remote linux machine
-...
The questions this addresses are:
- How can I efficiently download my data from Galaxy once I've completed my analyses?
- How do I delete old data to make more room in my quota?
\nThe objectives are:
- Have a greater knowledge of how Galaxy handles data downloads and deletions
- Be able to...
The questions this addresses are:
- I have short reads and long reads. How do I assemble a genome?
\nThe objectives are:
- Perform Quality Control on your reads
- Perform a Small genome Assembly with Unicycler
- Evaluate the Quality of the Assembly with Quast
- Annotate the assembly with...
The questions this addresses are:
- What are the factors that affect genome assembly?
- How does Genome assembly work?
\nThe objectives are:
- Perform an optimised Velvet assembly with the Velvet Optimiser
- Compare this assembly with those we did in the basic tutorial
- Perform an...
The objectives are:
- Have an understanding of what CVMFS is and how it works
- Install and configure the CVMFS client on a linux machine and mount the Galaxy reference data repository
- Configure your Galaxy to use these reference genomes and indices
- Use an ansible playbook for all of the...
The questions this addresses are:
- How does pulsar work?
- How can I deploy it?
\nThe objectives are:
- Have an understanding of what Pulsar is and how it works
- Install and configure a Pulsar server on a remote linux machine
- Be able to get Galaxy to send jobs to a remote Pulsar server
The questions this addresses are:
- How to monitor Galaxy with Telegraf
- How do I set up InfluxDB
- How can I make graphs in Grafana?
- How can I best alert on important metrics?
\nThe objectives are:
- Setup InfluxDB
- Setup Telegraf
- Setup Grafana
- Create several charts