Variant Analysis - Calling very rare variants
Exome sequencing means that all protein-coding genes in a genome are sequenced
Questions of the tutorial:
- What frequency of variants is so low that it is obscured by sequencing error rate?
- What are the different types of consensus sequences produced from duplex sequencing?
Objectives of the tutorial:
- Processing raw duplex sequencing data into consensus sequences
- Find rare variants without relying on diploid assumptions
Resource type: Tutorial