Transcriptomics - Differential abundance testing of small RNAs

Training material for all kinds of transcriptomics analysis.

Questions of the tutorial:

  • What small RNAs are expressed?
  • What RNA features have significantly different numbers of small RNAs targeting them between two conditions?

Objectives of the tutorial:

  • Process small RNA-seq datasets to determine quality and reproducibility.
  • Filter out contaminants (e.g. rRNA reads) in small RNA-seq datasets.
  • Differentiate between subclasses of small RNAs based on their characteristics.
  • Identify differently abundant small RNAs and their targets.


Additional information

Resource type: Tutorial

Authors: malloryfreeberg