slides

Slides for 'Nucleoli Segmentation <br> & <br> Feature Extraction <br> using CellProfiler' tutorial

The questions this addresses are:
- How do I run an image analysis pipeline on public data using CellProfiler?
- How do I analyse the DNA channel of fluorescence siRNA screens?
- How do I download public image data into my history?
- How do I segment and label cell nuclei?
- How do I segment nucleoli (as the absence of DNA)?
- How do I combine nuclei and nucleoli into one segmentation mask?
- How do I extract the background of an image?
- How do I relate the nucleoli to their parent nucleus?
- How do I measure the image and object features?
- How do I measure the image quality?

The objectives are:
- How to download images from a public image repository.
- How to segment cell nuclei using CellProfiler in Galaxy.
- How to segment cell nucleoli using CellProfiler in Galaxy.
- How to extract features for images, nuclei and nucleoli.

Licence: Creative Commons Attribution 4.0 International

Keywords: imaging

Target audience: Students

Resource type: slides

Authors: Beatriz Serrano-Solano

Contributors: Beatriz Serrano-Solano


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