hands-on tutorial
Hands-on for 'Inferring trajectories using Monocle3' tutorial
The questions this addresses are:
- How can I prepare input files for Monocle starting from an AnnData object?
- How can I infer lineage relationships between clusters, without a time series?
- What can trajectory analysis tell us?
The objectives are:
- Identify which operations to perform on an AnnData object to obtain the files needed for Monocle
- Follow the Monocle3 workflow and choose the right parameter values
- Compare the outputs from Scanpy and Monocle
- Interpet trajectory analysis results
Licence: Creative Commons Attribution 4.0 International
Keywords: single-cell, single-cell, 10x, paper-replication, transcriptomics
Target audience: Students
Resource type: hands-on tutorial
Scientific topics: Transcriptomics
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