hands-on tutorial

Hands-on for 'Inferring trajectories using Monocle3' tutorial

The questions this addresses are:
- How can I prepare input files for Monocle starting from an AnnData object?
- How can I infer lineage relationships between clusters, without a time series?
- What can trajectory analysis tell us?

The objectives are:
- Identify which operations to perform on an AnnData object to obtain the files needed for Monocle
- Follow the Monocle3 workflow and choose the right parameter values
- Compare the outputs from Scanpy and Monocle
- Interpet trajectory analysis results

Licence: Creative Commons Attribution 4.0 International

Keywords: single-cell, single-cell, 10x, paper-replication, transcriptomics

Target audience: Students

Resource type: hands-on tutorial

Scientific topics: Transcriptomics


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