hands-on tutorial
Hands-on for 'Pre-processing of Single-Cell RNA Data' tutorial
The questions this addresses are:
- What is single-cell?
- How do we process a batch?
- How do we process multiple batches?
- How do we check for cross-contamination?
The objectives are:
- Demultiplex FASTQ data via UMI-tools
- Understand and validate the extraction of barcodes
- Obtain an overview of general alignment and quantification techniques
- Generate a count matrix for downstream single-cell RNA analysis
Licence: Creative Commons Attribution 4.0 International
Keywords: single-cell, single-cell
Target audience: Students
Resource type: hands-on tutorial
Contributors: Anika Erxleben, Bérénice Batut, Mehmet Tekman, Wolfgang Maier
Scientific topics: Transcriptomics
Activity log
