hands-on tutorial
Hands-on for 'Library Generation for DIA Analysis' tutorial
The questions this addresses are:
- How does the peptide identification work in data independent acquisition (DIA) mass spectrometry (MS) data?
- What is a spectral library and how to generate a spectral library using Maxquant, diapysef and OpenSwath tools?
The objectives are:
- Generating a spectral library from data dependent acquisition (DDA) MS data
- Understanding DIA data principles and characteristics
- Optimizing and refining a spectral library for the analysis of DIA data
Licence: Creative Commons Attribution 4.0 International
Keywords: proteomics, DIA
Target audience: Students
Resource type: hands-on tutorial
Contributors: Matthias Fahrner, Melanie Föll
Scientific topics: Proteomics
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