hands-on tutorial
Hands-on for 'Identification of the binding sites of the T-cell acute lymphocytic leukemia protein 1 (TAL1)' tutorial
The questions this addresses are:
- How is raw ChIP-seq data processed and analyzed?
- What are the binding sites of TAL1?
- Which genes are regulated by TAL1?
The objectives are:
- Inspect read quality with FastQC
- Perform read trimming with Trimmomatic
- Align trimmed reads with BWA
- Assess quality and reproducibility of experiments
- Identify TAL1 binding sites with MACS2
- Determine unique/common TAL1 binding sites from G1E and Megakaryocytes
- Identify unique/common TAL1 peaks occupying gene promoters
- Visually inspect TAL1 peaks with Trackster
Licence: Creative Commons Attribution 4.0 International
Keywords: epigenetics, ChIP-seq
Target audience: Students
Resource type: hands-on tutorial
Contributors: Anika Erxleben, Joachim Wolff, Mallory Freeberg, Mo Heydarian, Vivek Bhardwaj
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