hands-on tutorial

Hands-on for 'Downstream Single-cell RNA analysis with RaceID' tutorial

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The questions this addresses are:
- What is normalisation and why is it necessary?
- How many types of unwanted variation are there?
- How are biological phenotypes clustered?
- What is the difference between PCA and tSNE?
- What is the difference between cell trajectory and cell fate?

The objectives are:
- Filtering, normalising, and clustering cells in a matrix
- Assessing the quality of individual clusters
- Inferring cell type lineages
- Examining gene expression
- Determining the top most expressive genes per cluster
- Correcting for unwanted variation

Licence: Creative Commons Attribution 4.0 International

Keywords: single-cell, single-cell

Target audience: Students

Resource type: hands-on tutorial

Authors: Alex Ostrovsky, Mehmet Tekman

Contributors: Alex Ostrovsky, Mehmet Tekman

Scientific topics: Transcriptomics

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