hands-on tutorial
Hands-on for 'Creating the single-cell RNA-seq reference dataset for deconvolution' tutorial
The questions this addresses are:
- Where can I find good quality scRNA-seq reference datasets?
- How can I reformat and manipulate these downloads to create the right format for MuSiC?
The objectives are:
- You will retrieve raw data from the EMBL-EBI Single cell expression atlas.
- You will manipulate the metadata and matrix files.
- You will combine the metadata and matrix files into an ESet object for MuSiC deconvolution.
- You will create multiple ESet objects - both combined and separated out by disease phenotype for your single cell reference.
Licence: Creative Commons Attribution 4.0 International
Keywords: single-cell, single-cell, human, deconvolution, bulk, transcriptomics
Target audience: Students
Resource type: hands-on tutorial
Contributors: Mehmet Tekman, Wendi Bacon
Activity log
